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rabbit anti pkcε  (Proteintech)


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    Structured Review

    Proteintech rabbit anti pkcε
    Rabbit Anti Pkcε, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+pkc%CE%B5/PKC+epsilon+Antibody/pm41927530-440-96-100
    Average 93 stars, based on 14 article reviews
    rabbit anti pkcε - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Circulating extracellular microRNAs in the blood promote sociability in mice
    Article Snippet: Free floating coronal sections (40 μm in thickness) were prepared with a Leica cryostat and, if necessary, antigen retrieval was performed with 10 mM sodium citrate buffer (pH8.5). .. The sections were then placed in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 0.1% TritonX, 0.05% Tween-20, and 0.05% sodium azide) for 1 h at room temperature and then incubated at 4°C overnight with the following primary antibodies: mouse anti-NeuN (1:500, #MAB377, Merck-Millipore, RRID: AB_2298772), rabbit anti-Iba1 (1:400, #019-19741, Wako Chemicals, RRID: AB_839504), goat anti-c-Fos (1:500, #sc-52, Santa Cruz Biotechnology, RRID: AB_2106783), rabbit anti-c-Fos (1:200, #9F6, Cell Signaling, RRID: AB_22472111), mouse anti-CaMKIIα (1:200, #688602, Clone 6G9, BioLegend, RRID: AB_2617027), rabbit anti-CaMKIIα (1:100, #20666, Proteintech, RRID:AB_2878722), rabbit anti-RFP (1:1,000, #600-401-379, Rockland, RRID: AB_2209751), rabbit anti-PKCε (1:100, #20877-1-AP, Proteintech, RRID:AB_10697812), mouse anti-CC1 (1:100, #OP80, Calbiochem, RRID:AB_2057371), rabbit anti-S100β (1:200, #ab868, Abcam, RRID:AB_306716), mouse anti-NSE (1:500, #66150, Proteintech, RRID:AB_2881546), mouse anti-Gephyrin (1:100, #147021, Synaptic Systems, RRID:AB_2232546), and rabbit anti-GABAARγ2 (1:100, #AGA-005, Alomone Labs). .. After washing with PBS, the sections were further incubated with fluorophore-conjugated secondary antibodies at 1:400 dilution for 2 h at room temperature, followed by DAPI staining (1:50,000, #10236276001, Roche) for 10 min at room temperature. were used for signal detection.

    Article Title: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.
    Article Snippet: Free floating coronal sections (40 μm in thickness) were prepared with a Leica cryostat and, if necessary, antigen retrieval was performed with 10 mM sodium citrate buffer (pH8.5). .. The sections were then placed in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 0.1% TritonX, 0.05% Tween-20, and 0.05% sodium azide) for 1 h at room temperature and then incubated at 4°C overnight with the following primary antibodies: mouse anti-NeuN (1:500, #MAB377, Merck-Millipore, RRID: AB_2298772), rabbit anti-Iba1 (1:400, #019-19741, Wako Chemicals, RRID: AB_839504), goat anti-cFos (1:500, #sc-52, Santa Cruz Biotechnology, RRID: AB_2106783), rabbit anti-c-Fos (1:200, #2250, Cell Signaling, RRID: AB_22472111), mouse anti-CaMKIIα (1:200, #688602, Clone 6G9, BioLegend, RRID: AB_2617027), rabbit anti-CaMKIIα (1:100, #20666-1-AP, Proteintech, RRID:AB_2878722), rabbit anti-RFP (1:1,000, #600-401-379, Rockland, RRID: AB_2209751), rabbit anti-PKCε (1:100, #20877-1-AP, Proteintech, RRID:AB_10697812), mouse anti-CC1 (1:100, #OP80, Calbiochem, RRID:AB_2057371), rabbit anti-S100β (1:200, #ab868, Abcam, RRID:AB_306716), mouse anti-NSE (1:500, #66150-1-g, Proteintech, RRID:AB_2881546), mouse anti-Gephyrin (1:100, #147021, Synaptic Systems, RRID:AB_2232546), and rabbit anti-GABAARγ2 (1:100, #AGA-005, Alomone Labs, RRID: AB_2039870). .. After washing with PBS, the sections were further incubated with fluorophoreconjugated secondary antibodies at 1:400 dilution for 2 h at room temperature, followed by DAPI staining (1:50,000, #10236276001, Roche) for 10 min at room temperature. were used for signal detection.

    Incubation:

    Article Title: Circulating extracellular microRNAs in the blood promote sociability in mice
    Article Snippet: Free floating coronal sections (40 μm in thickness) were prepared with a Leica cryostat and, if necessary, antigen retrieval was performed with 10 mM sodium citrate buffer (pH8.5). .. The sections were then placed in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 0.1% TritonX, 0.05% Tween-20, and 0.05% sodium azide) for 1 h at room temperature and then incubated at 4°C overnight with the following primary antibodies: mouse anti-NeuN (1:500, #MAB377, Merck-Millipore, RRID: AB_2298772), rabbit anti-Iba1 (1:400, #019-19741, Wako Chemicals, RRID: AB_839504), goat anti-c-Fos (1:500, #sc-52, Santa Cruz Biotechnology, RRID: AB_2106783), rabbit anti-c-Fos (1:200, #9F6, Cell Signaling, RRID: AB_22472111), mouse anti-CaMKIIα (1:200, #688602, Clone 6G9, BioLegend, RRID: AB_2617027), rabbit anti-CaMKIIα (1:100, #20666, Proteintech, RRID:AB_2878722), rabbit anti-RFP (1:1,000, #600-401-379, Rockland, RRID: AB_2209751), rabbit anti-PKCε (1:100, #20877-1-AP, Proteintech, RRID:AB_10697812), mouse anti-CC1 (1:100, #OP80, Calbiochem, RRID:AB_2057371), rabbit anti-S100β (1:200, #ab868, Abcam, RRID:AB_306716), mouse anti-NSE (1:500, #66150, Proteintech, RRID:AB_2881546), mouse anti-Gephyrin (1:100, #147021, Synaptic Systems, RRID:AB_2232546), and rabbit anti-GABAARγ2 (1:100, #AGA-005, Alomone Labs). .. After washing with PBS, the sections were further incubated with fluorophore-conjugated secondary antibodies at 1:400 dilution for 2 h at room temperature, followed by DAPI staining (1:50,000, #10236276001, Roche) for 10 min at room temperature. were used for signal detection.

    Article Title: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.
    Article Snippet: Free floating coronal sections (40 μm in thickness) were prepared with a Leica cryostat and, if necessary, antigen retrieval was performed with 10 mM sodium citrate buffer (pH8.5). .. The sections were then placed in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 0.1% TritonX, 0.05% Tween-20, and 0.05% sodium azide) for 1 h at room temperature and then incubated at 4°C overnight with the following primary antibodies: mouse anti-NeuN (1:500, #MAB377, Merck-Millipore, RRID: AB_2298772), rabbit anti-Iba1 (1:400, #019-19741, Wako Chemicals, RRID: AB_839504), goat anti-cFos (1:500, #sc-52, Santa Cruz Biotechnology, RRID: AB_2106783), rabbit anti-c-Fos (1:200, #2250, Cell Signaling, RRID: AB_22472111), mouse anti-CaMKIIα (1:200, #688602, Clone 6G9, BioLegend, RRID: AB_2617027), rabbit anti-CaMKIIα (1:100, #20666-1-AP, Proteintech, RRID:AB_2878722), rabbit anti-RFP (1:1,000, #600-401-379, Rockland, RRID: AB_2209751), rabbit anti-PKCε (1:100, #20877-1-AP, Proteintech, RRID:AB_10697812), mouse anti-CC1 (1:100, #OP80, Calbiochem, RRID:AB_2057371), rabbit anti-S100β (1:200, #ab868, Abcam, RRID:AB_306716), mouse anti-NSE (1:500, #66150-1-g, Proteintech, RRID:AB_2881546), mouse anti-Gephyrin (1:100, #147021, Synaptic Systems, RRID:AB_2232546), and rabbit anti-GABAARγ2 (1:100, #AGA-005, Alomone Labs, RRID: AB_2039870). .. After washing with PBS, the sections were further incubated with fluorophoreconjugated secondary antibodies at 1:400 dilution for 2 h at room temperature, followed by DAPI staining (1:50,000, #10236276001, Roche) for 10 min at room temperature. were used for signal detection.



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    FIGURE 3 High-fat diet (HFD) supplemented with medium-chain triglycerides (MCTs) reduces hepatic diacylglycerol and ceramide content. (a, b) Liver ceramide and diacylglycerol (DAG) content. (c) Representative Western blot bands along with quantification (bottom) showing protein kinase C-ε <t>(PKCε)</t> protein expression level <t>in</t> <t>membrane</t> (Memb) normalized to Na+,K+-ATPase intensity, cytoplasmic (Cyto) normalized to heat shock protein 90 (HSP90) intensity, and whole liver protein kinase C-ζ (PKCζ) protein expression normalized to GAPDH. (d) Schematic representation of de novo ceramide synthesis pathway (light yellow box), sphingomyelin hydrolysis pathway (light blue box) and salvage pathway (light pink box). This schematic diagram was created using BioRender. (e) Hepatic expression of mRNAs encoded by de novo ceramide synthesis-related genes. (f) Hepatic expression of mRNAs encoded by sphingomyelin hydrolysis-related genes. (g) Hepatic expression of mRNAs encoded by salvage pathway-related genes. In scatter plots with a bar, one circle represents data from one mouse, and n = 6–8 mice per group. Values are presented as means ± SD. Statistical significance was determined using one-way ANOVA. *P < 0.05, HFD + MCTs group versus HFD group. †P < 0.05, HFD-to-LFD group versus HFD group. Abbreviations: Asah1–3, acid ceramidase 1–3; Cers1–6, ceramide synthase 1–6; Degs1 and 2, dihydroceramide desaturase 1 and 2; Sgms1 and 2, sphingomyelin synthase 1 and 2; Smpd1–3, sphingomyelin phosphodiesterase 1–3; Sptlc1 and 2, serine palmitoyltransferase long chain base subunit 1 and 2.
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    Image Search Results


    FIGURE 3 High-fat diet (HFD) supplemented with medium-chain triglycerides (MCTs) reduces hepatic diacylglycerol and ceramide content. (a, b) Liver ceramide and diacylglycerol (DAG) content. (c) Representative Western blot bands along with quantification (bottom) showing protein kinase C-ε (PKCε) protein expression level in membrane (Memb) normalized to Na+,K+-ATPase intensity, cytoplasmic (Cyto) normalized to heat shock protein 90 (HSP90) intensity, and whole liver protein kinase C-ζ (PKCζ) protein expression normalized to GAPDH. (d) Schematic representation of de novo ceramide synthesis pathway (light yellow box), sphingomyelin hydrolysis pathway (light blue box) and salvage pathway (light pink box). This schematic diagram was created using BioRender. (e) Hepatic expression of mRNAs encoded by de novo ceramide synthesis-related genes. (f) Hepatic expression of mRNAs encoded by sphingomyelin hydrolysis-related genes. (g) Hepatic expression of mRNAs encoded by salvage pathway-related genes. In scatter plots with a bar, one circle represents data from one mouse, and n = 6–8 mice per group. Values are presented as means ± SD. Statistical significance was determined using one-way ANOVA. *P < 0.05, HFD + MCTs group versus HFD group. †P < 0.05, HFD-to-LFD group versus HFD group. Abbreviations: Asah1–3, acid ceramidase 1–3; Cers1–6, ceramide synthase 1–6; Degs1 and 2, dihydroceramide desaturase 1 and 2; Sgms1 and 2, sphingomyelin synthase 1 and 2; Smpd1–3, sphingomyelin phosphodiesterase 1–3; Sptlc1 and 2, serine palmitoyltransferase long chain base subunit 1 and 2.

    Journal: Experimental Physiology

    Article Title: A high‐fat diet supplemented with medium‐chain triglycerides ameliorates hepatic steatosis by reducing ceramide and diacylglycerol accumulation in mice

    doi: 10.1113/ep091545

    Figure Lengend Snippet: FIGURE 3 High-fat diet (HFD) supplemented with medium-chain triglycerides (MCTs) reduces hepatic diacylglycerol and ceramide content. (a, b) Liver ceramide and diacylglycerol (DAG) content. (c) Representative Western blot bands along with quantification (bottom) showing protein kinase C-ε (PKCε) protein expression level in membrane (Memb) normalized to Na+,K+-ATPase intensity, cytoplasmic (Cyto) normalized to heat shock protein 90 (HSP90) intensity, and whole liver protein kinase C-ζ (PKCζ) protein expression normalized to GAPDH. (d) Schematic representation of de novo ceramide synthesis pathway (light yellow box), sphingomyelin hydrolysis pathway (light blue box) and salvage pathway (light pink box). This schematic diagram was created using BioRender. (e) Hepatic expression of mRNAs encoded by de novo ceramide synthesis-related genes. (f) Hepatic expression of mRNAs encoded by sphingomyelin hydrolysis-related genes. (g) Hepatic expression of mRNAs encoded by salvage pathway-related genes. In scatter plots with a bar, one circle represents data from one mouse, and n = 6–8 mice per group. Values are presented as means ± SD. Statistical significance was determined using one-way ANOVA. *P < 0.05, HFD + MCTs group versus HFD group. †P < 0.05, HFD-to-LFD group versus HFD group. Abbreviations: Asah1–3, acid ceramidase 1–3; Cers1–6, ceramide synthase 1–6; Degs1 and 2, dihydroceramide desaturase 1 and 2; Sgms1 and 2, sphingomyelin synthase 1 and 2; Smpd1–3, sphingomyelin phosphodiesterase 1–3; Sptlc1 and 2, serine palmitoyltransferase long chain base subunit 1 and 2.

    Article Snippet: The membrane was incubated overnight at 4◦C with antibodies against PKCε (1:1000, Cell Signaling, catalogue number 2683S), Na+,K+-ATPase (1:1000, Cell Signaling, catalogue number 3010S) and heat shock protein 90 (HSP90; 1:2000, BD Biosciences, catalogue no. 610418).

    Techniques: Western Blot, Expressing, Membrane